Role of potassium solubilizing microbes in disease and stress resistance in crops. Isolation and characterization of phosphate solubilizing. Number of microbial species plays key role in p solubilization these includes bacteria, fungi, actinomycetes and even algae. These potassiumsolubilizing bacteria ksb can be used as a promising approach to increase k availability in soils, thus playing an important role for crop establishment under klimited soils. Isolation and identification of two potassium solubilizing. Zinc is key micronutrient plays vital roles in plant metabolic processes.
Potassiumsolubilizing bacteria ksb can improve soil fertility and plant growth as biofertilizers by decomposing silicate minerals and releasing insoluble k into soluble forms. Towards the soil sustainability and potassium solubilizing microorganisms. The integrated use of rock phosphate and psm add 2030 kg p2o5ha. Also the fungal genera with this capacity are penicillium and aspergillu. Inoculation of phosphate solubilizing micro organisms increase crop yield by 510 %. Case study of improvement of soil condition for phosphate solubilizers on the fiftyyears longterm experiment in rice paddy soils in korea a considerably higher concentration of phosphate solubilizing bacteria is commonly found in the rhizosphere soil. Aleksandrow agar m1996 for isolation and detection of potassium solubilizing bacteria from soil samples. Potassium solubilizing microorganisms and their role in enhancing soil fertility and health.
Isolation and identification of potassiumsolubilizing. Plants are exposed to many abiotic and biotic stresses such as chilling, drought, high light intensity, nutrient limitations, heat, diseases, reactive oxygen species hydroxyl radical oh. They are gaining importance because of ecofriendly, nonhazardous, and nontoxic nature kannaiyan et al. Characteristics and biodiversity of endophytic phosphorus. Ksolubilization is carried out by a large number of saprophytic bacteria bacillus mucilaginosus, b. Large resources of good quality feldspar 810% potassium in rajasthan, india, could also be effectively utilized by native potassium solubilizing microorganisms. Potassium is a vital component of plant nutrition package limiting crop yield and. Potassium is one of the essential nutrients for plant to growth and required in large amount to achieve optimum growth. Five ksb, isolated from banana and chilli rhizosphere were identified by 16s rdna sequence analysis. A complex inoculant of n2fixing, p and ksolubilizing bacteria from a purple soil improves the growth of kiwifruit actinidia chinensis.
Phosphate solubilizing biofertilizers as a component of inm many heterotropjic bacteria and fungi efficiently solubilize insoluble phosphate of soil and rock phosphates. Most of these isolates are aerobic, capsulated, motile and 2 of them are gram positive while 3 are gram negative. Seven bacteria isolates capable of solubilizing potassium were ljk 1, ljk 2, ljk 4, lbk 4, lbk 5, psuk 4, and psik 6. Potassium solubilizing bacterial strains resulted in release of 35. Twenty seven bacterial isolates capable of solubilizing potassium were isolated from. A field experiment was conducted to study the efficacy of indigenous potassium solubilizing bacteria. Based on the potassium solubilizing index, seventh isolates were capable of solubilizing feldspar in alexandrov solid medium, the best isolate with the highest score was ljk 2 5,25 cm in diameter, contrary with the lowest. Characteristics of endophytic p and k solubilizing bacteria 451 acta biologica hungarica 66, 2015 5. Fundamentally, potassium is a macronutrient in plants.
Composition ingredients gms litre dextrose glucose 10. Comparative efficiency of five potash and phosphate. Phosphate solubilization all of the isolated bacterial isolates were checked for phosphate solubilization on pikoviskaya agar plates 1618. Biofertilizer is a good platform to deliver this primary macronutrient by assistance of potassium solubilizing bacteria ksb. Potassium solubilizing bacteria ksb could serve as inoculants. Owing to naturally available source of potassium in soil and high price of synthetic potassium fertilizers, the importance of ksb is increasing day by day. The potassium solubilizing microorganisms ksms are a rhizospheric microorganism which solubilizes the insoluble potassium k to soluble forms of k for plant growth and yield. Phosphate and potassium are major essential macronutrients for plant growth and development and soluble p and k fertilizers are commonly applied to re.
Paper open access isolation and characterization of. Potassium solubilizing bacteria and their application in agriculture. Isolation and screening of silicate bacteria from various. Asam oksalat, asam sitrat, asam tartarat, asam format dan asam malat merupakan asamasam organik yang diduga dihasilkan dari proses. Pdf potassium solubilizing bacteria ksb researchgate. Potassium solubilizing bacteria ksb such as bacillus mucilagenosus and bacillus edaphicus are example of microorganisms that used in biofertilizer. To acquire the optimum outcome by applying this bacterium into the. Isolation and characterization of potassium solubilizing bacteria from paddy rhizosphere oryza sativa l. Twenty endophytic phosphorus and potassium solubilizing bacteria were screened from 82 bamboo plants, among which the ctb092, wysa011 and jlb06. The potassium solubilizing microorganisms ksms are a rhizospheric.
Symbionk is a biofertilizer based on a selective strain of potash solubilizing, beneficial bacteria frateuria aurantia. Prajapati and others published isolation and characterization of potassium solubilizing bacteria from ceramic industry soil find, read and cite all the research you. Percentage of silicate solubilizing bacteria was checked for each soil sample and calculated as follows. Potassium solubilizing microorganisms for sustainable. Sneath ed bergeys manual of systematic bacteriology, vol 2. These strains were evaluated for their ability to solubilize the fixed k from waste biotite wb. Potassiumsolubilizing bacteria and their application in. The morphological and the biochemical characteristics of the 5 good potassium solubilizers are presented in tables 2. The utility of microorganisms for solubilizing the unavailable forms of potassium k from soil has led to renewed interest in fabrication of rapid and sensitive plate assays for their isolation and screening. A complex inoculant of n2fixing, p and ksolubilizing. Isolation of potassium solubilizing bacteria a total of 37 potassium solubilizing bacteria isolates were obtained from limestone quarry area soil samples of pt indocement tbk, palimanan cirebon using aleksandrov medium. This procedure was repeated five times and the obtained suspensions from each plot were mixed and then diluted with. Potassium solubilisation and identification of best solubilizer the all five isolates were grown on k enriched 100 ml broth containing 1% glucose, 0.
Isolation of phosphate solubilizing bacteria from maize. Occurrence, mechanisms and their role in crop production ahmad ali khan 1, ghulam jilani 1, mohammad saleem akhtar 1, syed muhammad saqlan naqvi 2, mohammad rasheed 3 abstract plants acquire phosphorus from soil solution as phosphate anion. Potassium solubilizing microorganisms for sustainable agriculture. Suspension was spread on pikovskaya agar composition of 1 l medium. This is a promising strategy for the improvement of plant absorption of potassium and so reducing the use of chemical fertilizer. Phosphate solubilizing microorganisms naturally occurring rhizospheric phosphorus solubilizing microorganism psm dates back to 1903 khan et al. Badr41 studied potassium and phosphorus solubilization capacity of silicate solubilizing bacteria and it ranged from 490 mg to 758 mg l1 at ph 6. Endophytic phosphorus and potassium solubilizing bacteria were screened from the root, rhizome, stem, and leaves of moso bamboo, and their diversity was analyzed using their 16s rdna sequences.
Composition ingredients gms litre magnesium sulphate 0. Molecular characterization of potassium solubilizing. Ksolubilization is carried out by a large number of saprophytic bacteria. Many bacteria such as acidothiobacillus ferrooxidans, paenibacillus spp. Potassium uptake by crop as well as microorganisms. Indian conditions are tend to be deficient in zinc due to adverse climatic conditions, high ph and several other factors, whereas potassium is most important for plants in. These potassium solubilizing bacteria ksb were found to dissolve potassium, silicon and aluminium from insoluble kbearing minerals such as micas, illite and orthoclases, by excreting organic acids which either directly dissolved rock k or chelated silicon ions to bring k into the solution14 16. Phosphorus solubilizing bacteria play role in phosphorus nutrition by enhancing its availability to plants through release from inorganic and organic soil p pools by solubilization and mineralization. Therefore the present work was conducted to isolate and test feldspar. Zinc solubilizing agar m2023 intended use recommended for growth and maintenance of zinc solubilizing soil microorganisms. A complex inoculant of n2fixing, p and ksolubilizing bacteria. Pgp bacteria isolation and determinations of n 2fixing, p and k solubilizing ability. The potassium solubilizing microorganisms ksms are a rhizospheric microorganism.
However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Potassium solubilizing bacteria ksb, modified aleksandrov media, dilution plating, trial plots, germination rate. Isolation and characterization of potassiumsolubilizing. Effect of coinoculation with phosphate and potassium.
Differences in distribution of potassiumsolubilizing. The potassium solubilizing bacteria isolates were characterized by a clear zone that formed around the colony figure 1. Symbionk, biofertilizer, potash solubilizing, bacteria. Isolation, characterization and identification of potassium solubilizing bacteria from rhizosphere soil of maize zea mays. The present investigation comprises a total of fifty potassium solubilizing bacterial ksb strains which were isolated from oryza sativa, musa paradisiaca, zea mays, sorghum bicolor and triticum aestivum l. Isolation of phosphate solubilizing bacteria from maize rhizosphere and their potential for rock phosphate solubilizationmineralization and plant growth promotion. Beneficial bacteria activate nutrients and promote wheat.
Four 4 potassium solubilizing bacteria ksb, eight 8 zinc solubilizing bacteria znsb and two 2 zinc solubilizing fungi znsf were isolated from rice, maize, cotton and sorghum rhizosphere soils collected from student and college farm, rajendranagar, pjtsau, telangana. They convert insoluble potassium in the soil into a form that plants can access. These two efficient k solubilizing bacteria and one phosphorus solubilizing bacteria were examined for their performance on maize. Biochemical study and bergeys manual of systematic. The structure and functional activity of the photosynthetic apparatus should be investigated at different levels of its organization. These potassium solubilizing bacteria ksb can be used as a promising approach to increase k availability in soils, thus playing an important role for crop establishment under klimited soils. Inorganic phosphorus plate medium was made as follows.
Comparative efficiency of five potash and phosphate solubilizing bacteria and their key enzymes useful for enhancing and improvement of soil fertility sajidmohammad a saiyad, dr. Isolation and characterization of potassium solubilizing. Isolation and purification of potassium solubilizing bacteria from. Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil.
These microbial isolates showed potassium solubilization index from 1. Potassiumsolubilizing bacteria can also dissolve tricalcium phosphate in medium tables 1 and 2 and additional file 3. Of phosphate solubilizing bacteria and their coinoculation efficiency on tomato. Perspectives of potassium solubilizing microbes in. Characterization of potassium solubilizing bacteria ksb in. The cultures were incubated at room temperature for 4872 hours. The biochemical characters are studied for the five isolates of potassium solubilizing bacteria presented in. The utilization of potassium solubilizing bacteria ksb to increase the soluble k content in soil has been regarded as a desirable pathway to increase plant yields.